Human Giardia
FOR RESEARCH USE ONLY

Assay range：1.0 pg/mL - 40pg/mL 96 determinations

Purpose

This kit allows for the determination of Giardia concentrations in
Human serum, cell culture supernates and other biological fluids

Principle of the assay

The kit assay Human Giardia level in the sample, use Purified Human
Giardia antibody to coat microtiter plate wells, make solid-phase antibody, then
add Giardia to wells, Combined Giardia antibody which With HRP labeled,
become antibody - antigen - enzyme-antibody complex, after washing
Completely, Add TMB substrate solution,TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of Human Giardia in the samples is
then determined by comparing the O.D. of the samples to the standard curve.